www.plastid.no © 2007                    

 

Plant Transformation Made Simple

Advantages and Potential of Plastid Transformation:

 

Press pages - for the most current news items.

 

Links

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"chloroplast image makes front cover of the prestigious Embo Journal"

An alternative to nuclear transformation and complementary

methodology for plant modification.

 

 

Patented technology based on the research of Professor Simon Moller (St. Johns University, New York, CORE, UiS, Rockefeller University, Leicester University).
 This new plastid transformation technology employs standard biolistic delivery of transgenes to chloroplasts but has several advantages over conventional plastid transformation protocols:

  • Positive, hormone-based, selection system enabling more efficient selection of transformants,
  • Faster regeneration during initial stages,
  • Can be combined with negative selection to drive homoplasmy,
  • Has the potential to expand the range of target crops for trait modification or production of proteins/enzymes.

     

Feature

Nuclear

Plastid

Clean genetics

No

YES

Uniform expression

No

YES

Multiple stacking

Possible

YES

Silencing

Yes

NO

Repeatable

No

YES

Maternal inheritance

No

YES

Costly infrastructure

No

NO

Production costs

Low

LOW

Cost of scaling

Low

LOW

Applications:

Increased biomass, multiple trait engineering, biofuels, functional food/feed, therapeutics

 

  • Transformation of plastids in crops
  • Expression of ”difficult” recombinant proteins
  • Expression of antigens as edible therapeutics