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Technology
Basic principles of plastid transformation:
First, the gene or genes to be introduced into the plastid genome are coated onto microscopic gold particles (0.6-1 ìm in diameter). These DNA-coated gold-particles are then shot into tobacco cells using a helium-driven biolistic gun. Following shooting, transformed plant cells (plant cells that contain a plastid or plastids with the gene of interest) are selected and a new transplastomic plant is regenerated from this plant cell. Although simple in principle, the selection and regeneration of transplastomic plants is prone to errors using current conventional antibiotics-based selection methods. We have developed a new selection and regeneration method which is more efficient and reliable compared to currently available technologies.
| Current Plant Systems |
Plastid AS technology |
| - Based on antibiotics |
- Not based on antibiotics |
| - Slow selection (6 weeks) |
- Rapid selection (3 weeks) |
- Results in many spontaneous
mutations |
- No spontaneous mutations
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- Controlled protein production |
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- Produces active protein |
Plastid production will have similar
initial development costs for proteins
developed in yeast or bacterial
systems but very low production
costs. Relative purification costs are
the same for all systems. |
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Livreddende biotek ved UiS
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Med revolusjonerende metoder skal selskapet Plastid ASprodusere proteiner. Professor Simon Geir Møller står i spissen for selskapet, som er Universitetet i Stavangers første biotek-selskap.
Photos: Håkon Vold
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